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Prevalence Of Metallo-Β-Lactamase Producing Pseudomonas Spp.(In Tertiary Care Hospital)

Dr. Prajapati Sweta B.*, Dr. Oza Sunil G.**, Dr. Mehta Sanjay J.***, Dr. Vegad Mahendra M.****.

Abstract
Background: Metallo-β-lactamase (MBL) mediated resistance to carbapenem is an emerging threat in Pseudomonas isolates. The aim of this study is to detect metallo-β-lactamase producing isolates of Pseudomonas spp. from various clinical samples from indoor patients in a teaching hospital. Materials and Methods: Total 900 bacterial strains were isolated from different clinical samples from indoor patients. The bacterial strains were isolated and identified as per the standard guidelines. Amongst them 100 isolates of Pseudomonas were taken for the present study. All pseudomonas isolates were subjected to antimicrobial susceptibility testing by Kirby-Bauer disc diffusion method (CLSIs). In all imipenem resistant isolates of Pseudomonas spp., MBL detection was carried out by Imipenem-EDTA combined-disc synergy test (CDST). Results: Out of 100 isolates of Pseudomonas, 44 (44%) were imipenem resistant. Of these 44 isolates, 30 were producing MBL enzyme. 30 MBL positive isolate included 12 (40%) from surgical wards, 10 (33.33%) from tuberculosis ward, 4 (13%) from medicine ward, 2 (7%) from paediatric ward, 1 (3%) from urology ward and 1 (3%) from neonatal ICU. All MBL positive strains were resistant to β-lactams, aminoglycosides and fluoroquinolones. Conclusion: Prevalence of MBL producing Pseudomonas spp. is 30%. The MBL producing Pseudomonas spp. isolates were multidrug resistant. It is important to identify MBL producing pseudomonas isolates in laboratory as may cause serious infections and may cause a nosocomial outbreak. [Prajapati S et al NJIRM 2013; 4(2) : 68-70]

Key words: Carbapenem-imipenem, Metallo-β-lactamase, Pseudomonas spp.


 
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How to Cite this Article
Pubmed Style

B. DPS, G. DOS, J. DMS, M. DVM. Prevalence Of Metallo-Β-Lactamase Producing Pseudomonas Spp.(In Tertiary Care Hospital). NJIRM. 2013; 4(2): 68-70.


Web Style

B. DPS, G. DOS, J. DMS, M. DVM. Prevalence Of Metallo-Β-Lactamase Producing Pseudomonas Spp.(In Tertiary Care Hospital). http://www.nicpdjournals.com/?mno=36914 [Access: December 12, 2017].


AMA (American Medical Association) Style

B. DPS, G. DOS, J. DMS, M. DVM. Prevalence Of Metallo-Β-Lactamase Producing Pseudomonas Spp.(In Tertiary Care Hospital). NJIRM. 2013; 4(2): 68-70.



Vancouver/ICMJE Style

B. DPS, G. DOS, J. DMS, M. DVM. Prevalence Of Metallo-Β-Lactamase Producing Pseudomonas Spp.(In Tertiary Care Hospital). NJIRM. (2013), [cited December 12, 2017]; 4(2): 68-70.



Harvard Style

B., D. P. S., G., D. O. S., J., D. M. S. & M., D. V. M. (2013) Prevalence Of Metallo-Β-Lactamase Producing Pseudomonas Spp.(In Tertiary Care Hospital). NJIRM, 4 (2), 68-70.



Turabian Style

B., Dr. Prajapati Sweta, Dr. Oza Sunil G., Dr. Mehta Sanjay J., and Dr. Vegad Mahendra M.. 2013. Prevalence Of Metallo-Β-Lactamase Producing Pseudomonas Spp.(In Tertiary Care Hospital). National Journal of Integrated Research in Medicine, 4 (2), 68-70.



Chicago Style

B., Dr. Prajapati Sweta, Dr. Oza Sunil G., Dr. Mehta Sanjay J., and Dr. Vegad Mahendra M.. "Prevalence Of Metallo-Β-Lactamase Producing Pseudomonas Spp.(In Tertiary Care Hospital)." National Journal of Integrated Research in Medicine 4 (2013), 68-70.



MLA (The Modern Language Association) Style

B., Dr. Prajapati Sweta, Dr. Oza Sunil G., Dr. Mehta Sanjay J., and Dr. Vegad Mahendra M.. "Prevalence Of Metallo-Β-Lactamase Producing Pseudomonas Spp.(In Tertiary Care Hospital)." National Journal of Integrated Research in Medicine 4.2 (2013), 68-70. Print.



APA (American Psychological Association) Style

B., D. P. S., G., D. O. S., J., D. M. S. & M., D. V. M. (2013) Prevalence Of Metallo-Β-Lactamase Producing Pseudomonas Spp.(In Tertiary Care Hospital). National Journal of Integrated Research in Medicine, 4 (2), 68-70.



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